Genomes are characterized by large regions of homogeneous base compositions known as isochores. The latter are divided into GC-poor and GC-rich classes linked to distinct functional and structural properties. Several studies have addressed how isochores shape function and structure. To aid in this important subject, we present IsoXpressor, a tool designed for the analysis of the functional property of transcription within isochores. IsoXpressor allows users to process RNA-Seq data in relation to the isochores, and it can be employed to investigate any biological question of interest for any species. The results presented herein as proof of concept are focused on the preimplantation process in Homo sapiens (human) and Macaca mulatta (rhesus monkey).

GC level, isochores, preimplantation, RNA-Seq, transcription
Genome biology and evolution
Centrum Wiskunde & Informatica, Amsterdam, The Netherlands

Ayad, L.A.K, Dourou, A.-M, Arhondakis, S, & Pissis, S. (2020). IsoXpressor: A Tool to Assess Transcriptional Activity within Isochores. Genome biology and evolution, 12(9), 1573–1578. doi:10.1093/gbe/evaa171