Motivation: Third generation sequencing methods provide longer reads than second generation methods and have distinct error characteristics. While there exist many read simulators for second generation data, there is a very limited choice for third generation data. Results: We analyzed public data from Pacific Biosciences (PacBio) SMRT sequencing, developed an error model and implemented it in a new read simulator called SimLoRD. It offers options to choose the read length distribution and to model error probabilities depending on the number of passes through the sequencer. The new error model makes SimLoRD the most realistic SMRT read simulator available. Availability and Implementation: SimLoRD is available open source at and installable via Bioconda (

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Journal Bioinformatics
Grant This work was funded by the The Netherlands Organisation for Scientific Research (NWO); grant id nwo/639.072.309 - Statistical Models for Structural Genetic Variants in the Genome of the Netherlands
Stöcker, B.K. (Bianca K.), Köster, J, & Rahmann, S. (2016). SimLoRD: simulation of long read data. Bioinformatics, 32(17), 2704–2706. doi:10.1093/bioinformatics/btw286