We demonstrate the ability to record a tomographic tilt series containing 3487 images in only 3.5 s by using a direct electron detector in a transmission electron microscope. The electron dose is lower by at least one order of magnitude when compared with that used to record a conventional tilt series of fewer than 100 images in 15–60 minutes and the overall signal-to-noise ratio is greater than 4. Our results, which are illustrated for an inorganic nanotube, are important for ultra-low-dose electron tomography of electron-beam-sensitive specimens and real-time dynamic electron tomography of nanoscale objects with sub-ms temporal resolution.
Nature Publishing Group
Nature Scientific Reports
Three-Dimensional Reconstruction of Biological Specimen with Scanning Electron Microscopy
Scientific Computing

Migunov, V., Ryll, H., Zhuge, J., Simson, M., Strüder, L., Batenburg, J., … Dunin-Borkowski, R. (2015). Rapid low dose electron tomography using a direct electron detection camera. Nature Scientific Reports, 5, 14516:1–14516:5. doi:10.1038/srep14516